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Longevity & Aging · Diets

Do "longevity" proteins explain why eating less extends life?

The claim, precisely: sirtuins enables caloric-restriction lifespan extension

Leans support Longevity & Aging 🔬 Includes disconfirming
RefutedContestedStrong support
consensus score 0.30

Unclear — evidence is mixed, only in yeast and worms, and a careful study saw it largely vanish.

Evidence ladder

How far up the ladder this claim has climbed. A high consensus on a low rung means "consistent so far," not "proven in people."

Top evidence so far: Population patterns (Observational)

MechanismIn-vitroAnimalObservationalRCTMeta-analysis

How the studies fall

8 support 3 contradict 0 tested null 1 mixed · 12 sources, 10 independent groups

What the evidence shows

Foundational yeast/worm support (Guarente lab) that SIR2/SIRT1 mediate CR longevity, but the strong causal form is contested: Burnett 2011 found the prior overexpression effects collapse on controlled genetic backgrounds and DR can work independently of Sir2.

The evidence (12)

SourceGradeStanceQualityFinding
Wu LE
2023 · Aging Cell
animal contradicts moderate SIRT2 transgenic overexpression did NOT impact lifespan in normal mice (benefit only in a progeria model) - undercuts sirtuins as general longevity mediators.
Rogina B
2024 · Front Genet
observational mixed low Review by a sirtuin proponent: SIRT1/resveratrol links to CR and aging, but acknowledges contested resveratrol/SIRT1 lifespan data - net supportive-but-qualified.
Lin
2000 · Science
animal supports moderate CR-induced lifespan extension in yeast requires SIR2 and NAD synthesis (NPT1)
Yang H
2007 · Aging Cell
animal supports low Designed STAC compounds activating Sir2 extended yeast replicative lifespan, reinforcing the sirtuin-activation route to lifespan extension (in-vitro/yeast).
Maissan P
2021 · Biology (Basel)
observational supports low Systematic review: sirtuin activity extends lifespan across organisms via NAD+-dependent regulation at the metabolism/cell-cycle interface (supportive synthesis).
Tissenbaum
2001 · Nature
animal supports moderate Extra copy of sir-2.1 extends C. elegans lifespan up to 50%, upstream of daf-16
Qu Q
2025 · Nature
animal supports high Lithocholic acid (accumulated during CR) binds TULP3 to activate sirtuins and AMPK and slow ageing in mice - recent mechanistic support linking CR to sirtuin activation.
Lin SJ
2004 · Genes Dev
animal supports moderate CR extends yeast replicative lifespan by lowering NADH and activating the NAD-dependent deacetylase Sir2 - core mechanistic support for sirtuin-mediated CR longevity.
Wood
2004 · Nature
animal supports moderate Sirtuin-activating compounds (STACs) mimicked CR and delayed ageing in yeast, worms and flies in a Sir2-dependent manner, supporting sirtuins as CR effectors.
⚠️ correction-on-file (Crossref) - kept, corrigendum not retraction
Brenner C
2022 · Life Metab
observational contradicts moderate Argues sirtuins are NOT conserved longevity genes; cross-species lifespan claims fail the conservation test - directly contests the CR-sirtuin thesis.
Burnett
2011 · Nature
animal contradicts high With controlled genetic background, Sir2 overexpression does NOT extend lifespan in worms/flies; DR works independently of dSir2
Moroz N
2014 · Aging Cell
animal supports moderate Dietary restriction acted through NAD+-dependent (sirtuin) mechanisms and an oxidative-metabolism shift, supporting sirtuin involvement in DR longevity (independent lab).

Educational only, not medical advice. Grades and scores reflect published evidence weighted by study design and quality; see the methodology.